University of South Wales 2 articles published in JoVE Medicine Dual Test Gas Pulmonary Diffusing Capacity Measurement During Exercise in Humans Using the Single-Breath Method Stine B. Nymand1, Jacob Peter Hartmann1,2,3, Helene Louise Hartmeyer1, Iben E. Rasmussen1, Amalie Bach Andersen1, Milan Mohammad1, Susan Al-Atabi3, Birgitte Hanel3, Ulrik Winning Iepsen1,4, Jann Mortensen3,5, Ronan M. G. Berg1,2,3,6 1Centre for Physical Activity Research, Copenhagen University Hospital - Rigshospitalet, 2Department of Biomedical Sciences, Faculty of Health and Medical Sciences, University of Copenhagen, 3Department of Clinical Physiology and Nuclear Medicine, Copenhagen University Hospital - Rigshospitalet, 4Department of Anesthesiology and Intensive Care, Hvidovre Hospital, 5Department of Clinical Medicine, Faculty of Health and Medical Sciences, University of Copenhagen, 6Neurovascular Research Laboratory, Faculty of Life Sciences and Education, University of South Wales This protocol presents a method to assess pulmonary alveolar-capillary reserve measured by combined single-breath measurement of the diffusing capacity to carbon monoxide (DL,CO) and nitric oxide (DL,NO) during exercise. Assumptions and recommendations for using the technique during exercise form the foundation of this article. Medicine Doppler Ultrasound-Based Leg Blood Flow Assessment During Single-Leg Knee-Extensor Exercise in an Uncontrolled Setting Jacob Peter Hartmann1,2,3, Rikke Krabek1, Stine B. Nymand1,3, Helene Hartmeyer1, Lasse Gliemann4, Ronan M. G. Berg1,2,3,5, Ulrik Winning Iepsen1,6 1Centre for Physical Activity Research, Copenhagen University Hospital, 2Department of Clinical Physiology and Nuclear Medicine, Copenhagen University Hospital, 3Department of Biomedical Sciences, Faculty of Health and Medical Sciences, University of Copenhagen, 4Department of Nutrition, Exercise and Sports, Integrated Physiology Group, 5Neurovascular Research Laboratory, Faculty of Life Sciences and Education, University of South Wales, 6Department of Anaesthesiology and Intensive Care, Copenhagen University Hospital, Hvidovre Hospital This test-retest study evaluated leg blood flow measured by the Doppler ultrasound technique during single-leg knee-extensor exercise. The within-day, between-day, and inter-rater reliability of the method was investigated. The approach demonstrated high within-day and acceptable between-day reliability. However, the inter-rater reliability was unacceptably low during rest and at low workloads.
